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Meristem culture technique is an alternative technique without side-effects, which can be applied to endogenous contaminated explants. This technique is based on using the rapid growing meristematic cells natural mechanism without contacting the vascular tissue. Due to faster cell division than the development process of vascular tissue, the endogenous microbes in the vascular system cannot invade into the meristem area; therefore, the meristematic tissue is completely free of contamination.


Direct organogenesis is the crucial method to multiply the explants by getting the plant organs through the eliminating stock explants and sub-culturing onto the fresh medium. Repeated cycles of explants sub-culturing into different stages finally produce the necessary number of well-developed plantlets. The culturing techniques are created, and culture media are formulated through experiments to meet the growth requirements of explants and enhance lab production.


Callus induction of the plant tissue method, allows the R&D team to regenerate and multiply the explants in an alternative way. The explants form tissue clump, i.e, callus through auxin and cytokinin stimulation in culture media and adjustment of certain external factors. From the clump structure, the callus then separates and converts to buds, shoots, and eventually roots to be plantlets by manipulating the different stages of culture media and treatments.

Gain Green Development applies bioreactor on certain species’ micropropagation. The immersion and semi-immersion liquid culture system allow a larger number of explants to be produced in single culture cycle. The liquid medium agitated with filtered aeration increases the absorption of the explants nutrients. Compared to conventional solid medium culture, this method stimulates explants growth and multiplication rate within a shorter period.


DNA identification is also being performed. The identification process is implemented at the R&D lab for the true original species determination. The results obtained serve as the reference for our decision regarding further action toward the target plant.

To assure explants quality and eliminate contamination, bacteria and fungus identification is performed when persistent contamination is discovered in a particular culture. The targeted antimicrobial treatment is being applied to eliminate the contaminant in the culture and cut off the source of spreading.