Acclimatization of MD2 Pineapples in Nursery

Acclimatization of MD2 Pineapples in Nursery

The in vitro plantlet from tissue culture lab is not suitable to be planted immediately in the field. A physiological adaptation process called acclimatization is carried out in the nursery with a serial of hardening treatments to ensure the plantlets adapted through the stressful external environment before it can be planted in the field.

The ex-lab pineapple plantlets were transferred into the polybag containing planting substrates and supplemented with nutrients in order able to acclimatize through this transition period in the nursery. Under the monitored temperature, humidity and light, the plantlets developed into hardened seedlings after few weeks in the green house. During the acclimatization process, the leaf tissue of plantlet will grow thicker it will strengthen the whole leaf structure. An extracellular hydrophobic layer is also developed to cover aerial epidermis of plantlets providing protection against desiccation and external environmental stresses.

Gain Green Development’s laboratory offers high-quality tissue cultures to fulfil the customer’s demand. By Company’s team experts in keeping up protocols and creating new ones for untested plant species and varieties.

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Ananas comosus MD2 pineapple

Pineapple (Ananas comosus) is one of the most economically important tropical fruits within the Bromeliaceae family (Duval et al., 2001). It is the most highly traded tropical fruit. Pineapple is a perennial herbaceous and monocotyledonous flowering plant whereby it sends up a flower stalk from that central point. Generally, it is usually asymptomatic and has a range of height and width of 0.91 to 1.98 m (Bartholomew et al., 2003). In Malaysia, we have many varieties, namely Moris (AC1), Sarawak (AC2), Gandol (AC3), Maspine (AC4), Josapine (AC5), Yankee (AC6), Moris Gajah (AC7), N36 (AC8), MD2 (AC9), and many more. Among all, MD2 is the most favourite variety due to its super sweet content and yellowish flesh that commonly involved in food and beverage industry.

Top pineapple producing country is Costa Rica, followed by Philippines and Brazil. Malaysia produces about 330k tons annually. For the Southeast Asian region, Indonesia is among the third largest pineapple producers after the Philippines and Thailand with a contribution of around 23%. Pineapple is mostly consumed worldwide as fresh fruit due to its rich flavour, taste, and size. Consumed this way, it can be an excellent source of vitamin A, C, vitamin B1, vitamin B6, manganese, magnesium, iron, copper, phosphorus, and dietary fibre (“Fresh Pineapples,” 2008). It has no starch and consumers only need to peel, core, and slice the pineapple for consumption. Canned pineapple has a wide market as processed fruit. There are a great variety of the canned presentations such as whole, slices, or rings, fingers or spears, cubes, chunks, wedges, and tidbits.

Plain cans are used to pack the fruit because they do not contain tin plate which dissolves due to the fruit acid (Lobo & Paull, 2017). Sometimes the fruit is packed in syrup to hold its colour, shape, and flavour or it can be canned with water or fruit juice to reduce the sugar content. Other downstream products can be made from the pineapples are dried fruits, frozen pineapple, pineapple juice, pineapple jam, and meat tenderizer (bromelain). Pineapple leaf fibre is a hair-like material in elongated pieces similar to thread. The leaf fibre is a comprised mainly of cellulose (Bongarde & Shinde, 2014).

The seeds of these plants are very slow to germinate and therefore are not used for commercial purposes. This bromeliad is routinely propagated vegetatively by means of various vegetative parts, organs, or tissues such as:

  1. basal suckers originating from buds below ground level,

  2. hapas, which are shoots developing at the base of the peduncle,

  3. leafy branches arising from buds in leaf axils,

  4. slips, which grow out of the peduncle below or at the base of fruit,

  5. crowns, arising from the upper part of the fruit, and

  6. butts or stumps from the mature plant (Rangon, 1984).

In case of industrial scale production suckers and heaps are also used (Firoozabady and Gutterson, 2003). The tissue culture method of pineapple comes into the play to increase the selectivity of the desired traits coupled with a high multiplication rate. Following the standard tissue culture method (Mathews and Rangan, 1979; Zepeda and Segawa, 1981; Fitchet, 1990; Fitchet-Purnell, 1993; Kiss et al., 1995; Gangopadhyay et al., 2005) much higher rate of multiplication (40 to 85 fold in a 13 month period) was obtained.

Pineapple crops contaminated with viruses affect production and may lead to mealybug wilt of pineapple (MWP) disease which can be found in all pineapple crops in high density growing areas throughout the world (Sether et al. 2001). Mealybug wilt of pineapple symptoms are morphologically evident on plant leaves displaying leaf downward curling, reddening, wilting, or gradual dying of the tips (Sether et al., 2001; Sether et al., 2005).

Plant tissue culture is about one of the major option to develop an efficient and economical micropropagation protocol for the large scale propagation of pineapples. The micropropagation protocol has now become fairly standardized for some important cultivars of pineapple and with minor changes can be applied to newly derived ones. Propagation in temporary immersion bioreactors (Espinosa et al., 2002) which claims to be an improvement over the existing micropropagation protocol, reports a better performance of such plantlets over those propagated by conventional methods of micropropagation.


Bartholomew, D., Paull, R., & Rohrbach, K. (2003). The pineapple (pp. 1-291). CABI Publishing.

Bongarde, U. S., & Shinde, V. D. (2014). Review on natural fiber reinforcement polymer composties, 3.

Center for Agricultural Data and Information Systems Secretariat General of the Ministry of Agriculture 2016 Agricultural Commodities, Horticulture Sub Sector: Pineapple Outlook accessed on December 18th 2019

Duval MF, Noyer JL, Perrier X, Coppens d’Eeckenbrugge G, Hamon P (2001). Molecular diversity in pineapple assessed by RFLP markers. Theor. Appl. Genet. 102: 83-90.

Espinosa, P., Lorenzo, J.C., Iglesias, A., Yabor, L., Menendez, E., Borroto, J., Hernandez, L. & Arencibia, A.D. (2002) Production of pineapple transgenic plants assisted by temporary immersion bioreactors. Plant Cell Rep. 21, 136–140.

Firoozabady, E. and Gutterson, N. 2003. Cost effective in vitro propagation method for pineapple. Plant Cell Reproduction. 21: 844-850.

Fitchet, M. 1990. Clonal propagation of Queen and Smooth Cayenne pineapple. Acta Horticulturae. 275:261-266.

Fitchet-Purnell, M. 1993. Maximum utilization of pineapple crowns for micropropagation. Acta Horticulturae. 334: 325-330.

Fresh Pineapples. (2008). Retrieved April 1, 2017, from

Gangopadhyay, G., Bandyopadhyay, T., Poddar, R., Basu Gangopadhyay, S. and Mukherjee, K.K. 2005. Encapsulation of pineapple microshoots in alginate beads for temporary storage. Current Science. 88: 972-977.

Kiss, E., Kiss, J., Gyulai, G. and Heszky, L.E. 1995. A novel method for rapid micropropagation of pineapple. Horticulture Science. 30: 127-129.

Lobo, M. G., & Paull, R. E. (2017). Handbook of Pineapple Technology: Production, Postharvest Science, Processing and Nutrition. Wiley.

Mathews, H.V. and Rangan, T.S. 1979. Multiple plantlets in lateral bud and leaf explant in vitro cultures of pineapple. Scientia Horticlturae. 11: 319-328.

Rangan, T.S. 1984. Pineapple, p. 373–382. In: P.V. Ammirato, D.A. Evans, W.R. Sharp, and Y. Yamada (eds.). Handbook of plant cell culture 3, Crop species. Macmillan, New York.

Sether, D., A. Karasev, C. Okumura, C. Arakawa, F. Zee, M. Kislan, J. Busto, and J. Hu. 2001. Differentiation, Distribution, and Elimination of Two Different Pineapple mealybug wiltassociated viruses Found in Pineapple. Plant Disease 85:856-864.

Sether, D., M. Melzer, J. Busto, F. Zee, and J. Hu. 2005. Diversity and Mealybug Transmissibility of Ampeloviruses in Pineapple. Plant Disease 89:450-456.

Zepeda, C. and Sagawa, Y. 1981. In vitro propagation of Pineapple. Horticulture Science. 16:495.